HICE1 Polyclonal antibody

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• HICE1 Polyclonal antibody (33973-1-AP)

• 

  WB analysis using 33973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33973-1-AP (HICE1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 33973-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33973-1-AP (HICE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HICE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33973-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HAUS augmin-like complex subunit 8, HAUS8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48

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Tested Applications

• Positive WB detected in: Jurkat cells, MG-63 cells, U2OS cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33973-1-AP targets HICE1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag40571

  Product name: Recombinant human HICE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-284 aa of BC010176

  Sequence: DVGDSEENVQVLDLLSELKDVTAKKDLELRRSFAQVLELSAEASKEAALANQEVWEETQGMAPPSRWYFNQDSACRESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSRSGRDLS

• Full Name: HEC1/NDC80 interacting, centrosome associated 1
• Calculated Molecular Weight: 410 aa, 45 kDa
• Observed Molecular Weight: 38kDa, 45kDa
• GenBank Accession Number: BC010176
• Gene Symbol: HICE1
• Gene ID (NCBI): 93323
• RRID: AB_3743129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9BT25
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HICE1 protein (also known as HAUS8) is an important member of the HICE (Hippo pathway components-enriched) family. Hice1 shows distinct cytoplasmic localization and associates with interphase centrosomes and mitotic spindles, preferentially at the spindle pole vicinity. It primarily serves as a core component of the STRIPAK complex (Striatin-interacting phosphatase and kinase complex), where it inhibits the activity of the key kinases MST1/2 in the Hippo signaling pathway by regulating their dephosphorylation, thereby playing a critical role in organ size control, tissue homeostasis maintenance, and cell proliferation and apoptosis.