HIF-1 alpha Recombinant monoclonal antibody, PBS Only

Name: HIF-1 alpha Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 80933-1-PBS-100UG

HIF-1 alpha Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR

Cat No. 80933-1-PBS

Clone No. 2K1

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA, ChIP-qPCR

Synonyms

HIF1A, 2K1, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

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Filter: AllWBIF/ICCChIP-qPCRAffinity

WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 80933-1-RR (HIF1a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594+phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
ChIP experiment of HeLa using 80933-1-RR (same clone as 80933-1-PBS)
Chromatin was prepared from HeLa cells treated with cobalt chloride (100 μM, overnight). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of HIF-1 alpha (80933-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Chromatin was prepared from HeLa cells treated with cobalt chloride (100 μM, overnight). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of HIF-1 alpha (80933-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80933-1-RR
Biolayer interferometry (BLl) kinetic assays of 80933-1-RR against Human HIF-1 alpha were performed. The affinity constant is 0.235 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Product Information

80933-1-PBS targets HIF-1 alpha in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag15198 Product name: Recombinant human HIF1a protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 574-799 aa of BC012527 Sequence: LRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYD Predict reactive species
Full Name	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated Molecular Weight	826 aa, 93 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC012527
Gene Symbol	HIF1A
Gene ID (NCBI)	3091
RRID	AB_2918921
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16665
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).

Publications

What published studies show

The HIF-1 alpha Recombinant monoclonal antibody (2K1), catalog number 80933-1-PBS, has 1 indexed citation. It appears in Cell Death & Disease (2025), a journal focused on cell death and disease mechanisms. The cited study investigates ERCC6L-mediated stabilization of HIF-1α and its role in enhancing glycolysis and stemness properties of lung adenocarcinoma cells, placing the research in the fields of cancer metabolism and tumor biology.

All (1)

Species	Application	Title
		Cell Death Dis ERCC6L-mediated stabilization of HIF-1α enhances glycolysis and stemness properties of lung adenocarcinoma cells Authors - Zhimin Lu View Article