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HIF1AN Polyclonal antibody

HIF1AN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10646-1-AP

Synonyms

Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN



Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10646-1-AP targets HIF1AN in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF1AN fusion protein Ag0997
Full Name hypoxia inducible factor 1, alpha subunit inhibitor
Calculated molecular weight 40 kDa
GenBank accession numberBC007719
Gene symbol HIF1AN
Gene ID (NCBI) 55662
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1AN(Hypoxia-inducible factor 1-alpha inhibitor) is also named as FIH1. It is identified as a molecular oxygen-dependent dioxygenase that blunts the transcriptional activity of HIF and has also been implicated in HIF-dependent and -independent hypoxia responses(PMID:20720525). Structural studies indicate that an FIH-1 homodimer forms in solution and is essential for FIH-1 activity(PMID:16154531).

Protocols

Product Specific Protocols
WB protocol for HIF1AN antibody 10646-1-APDownload protocol
IHC protocol for HIF1AN antibody 10646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Bioact Mater

Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds.

Authors - Jinghuan Huang