|Positive WB detected in||HEK-293T cells, HeLa cells, HepG2 cells, rat brain tissue, rat kidney tissue|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21749-1-AP is HIGD1A Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HIGD1A fusion protein Ag14027|
|Full Name||HIG1 hypoxia inducible domain family, member 1A|
|Calculated molecular weight||93 aa, 10 kDa|
|Observed molecular weight||10 kDa|
|GenBank accession number||BC000601|
|Gene ID (NCBI)||25994|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.
Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
Mol Cell Proteomics
HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
Proc Natl Acad Sci U S A
Higd1a is a positive regulator of cytochrome c oxidase.