• Featured Product
  • KD/KO Validated

HIGD1A Polyclonal antibody

HIGD1A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21749-1-AP

Synonyms

HIG1, HIG1 domain family member 1A, HIGD1A



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21749-1-AP is HIGD1A Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIGD1A fusion protein Ag14027
Full Name HIG1 hypoxia inducible domain family, member 1A
Calculated molecular weight 93 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC000601
Gene symbol HIGD1A
Gene ID (NCBI) 25994
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

Protocols

Product Specific Protocols
WB protocol for HIGD1A antibody 21749-1-APDownload protocol
IHC protocol for HIGD1A antibody 21749-1-APDownload protocol
IF protocol for HIGD1A antibody 21749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

PLoS One

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.

Authors - Ameri Kurosh K
humanWB

Mol Cell Proteomics

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.

Authors - Daniella H Hock
  • KO Validated
humanWB, IF, IP

Proc Natl Acad Sci U S A

Higd1a is a positive regulator of cytochrome c oxidase.

Authors - Takaharu Hayashi
  • KD Validated