HIGD1A Polyclonal antibody

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  WB analysis using 21749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21749-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 21749-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 21749-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21749-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIGD1A antibody (21749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of A2780 using 21749-1-AP

  1x10^6 A2780 cells were intracellularly stained with 0.25 ug HIGD1A Polyclonal antibody (21749-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

• Featured Product
• KD/KO Validated

HIGD1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 21749-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

21749-1-AP targets HIGD1A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14027

  Product name: Recombinant human HIGD1A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-93 aa of BC000601

  Sequence: MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

• Full Name: HIG1 hypoxia inducible domain family, member 1A
• Calculated Molecular Weight: 93 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC000601
• Gene Symbol: HIGD1A
• Gene ID (NCBI): 25994
• RRID: AB_10858789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y241
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IF	Proc Natl Acad Sci U S A; Higd1a is a positive regulator of cytochrome c oxidase.; Authors - Takaharu Hayashi ; KD Validated
human	WB,IP,IF	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen ; KD Validated
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV; Authors - Daniella H Hock ; KO Validated
human	WB	FASEB J; Higd1a improves respiratory function in the models of mitochondrial disorder.; Authors - Takemasa Nagao
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.; Authors - Daniella H Hock ; KO Validated
	WB,IF	PLoS One; Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.; Authors - Ameri Kurosh K