HIGD1A Monoclonal antibody, PBS Only

HIGD1A Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A8E2

Cat no : 68231-1-PBS

Synonyms

DKFZp564K247, HIG1, HIG1 domain family member 1A, HIGD1A

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Validation Data Gallery

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  WB analysis using 68231-1-Ig (same clone as 68231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68231-1-Ig (HIGD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68231-1-Ig (same clone as 68231-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIGD1A antibody (68231-1-Ig, Clone: 3A8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68231-1-PBS targets HIGD1A in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIGD1A fusion protein Ag14027
• Full Name: HIG1 hypoxia inducible domain family, member 1A
• Calculated Molecular Weight: 93 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC000601
• Gene Symbol: HIGD1A
• Gene ID (NCBI): 25994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.