HIGD1B Polyclonal antibody

HIGD1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25367-1-AP

Synonyms

Protein CLST 11240, CLST11240 15, CLST11240

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Validation Data Gallery

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  IHC staining of human colon using 25367-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25367-1-AP (HIGD1B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat heart using 25367-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25367-1-AP (HIGD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: rat heart tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25367-1-AP targets HIGD1B in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIGD1B fusion protein Ag21871
• Full Name: HIG1 hypoxia inducible domain family, member 1B
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC020667
• Gene Symbol: HIGD1B
• Gene ID (NCBI): 51751
• RRID: AB_2880047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIG1 domain family member 1B (HIGD1B) is localized to the cell membrane whose expression is induced by hypoxia and glucose deprivation. HIG1 domain family member 1B (HIGD1B) is localized to the cell membrane whose expression is induced by hypoxia and glucose deprivation. (PMID: 34026765)