|Positive WB detected in||Jurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
10717-1-AP targets HINT1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HINT1 fusion protein Ag1142|
|Full Name||histidine triad nucleotide binding protein 1|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC007090|
|Gene ID (NCBI)||3094|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.
The role of the Hint1 protein in the metabolism of phosphorothioate oligonucleotides drugs and prodrugs, and the release of H2S under cellular conditions.
Biochim Biophys Acta
Interactions of cellular histidine triad nucleotide binding protein 1 with nucleoside 5'-O-monophosphorothioates and their derivatives - Implication for desulfuration process in the cell.
Int J Oncol
Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.
Distribution and expression of protein kinase C interactive protein (PKCI/HINT1) in mouse central nervous system (CNS).
Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.
Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.