HINT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HINT1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10717-1-AP

Synonyms

HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

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Validation Data Gallery

• 

  WB analysis using 10717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10717-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat thymus using 10717-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10717-1-AP

  IP result of anti-HINT1 (IP:10717-1-AP, 3ug; Detection:10717-1-AP 1:500) with mouse brain tissue lysate 7500ug.

• 

  IHC staining of human colon cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

10717-1-AP targets HINT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HINT1 fusion protein Ag1142
• Full Name: histidine triad nucleotide binding protein 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC007090
• Gene Symbol: HINT1
• Gene ID (NCBI): 3094
• RRID: AB_2248272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.

Publications

Species	Application	Title
human	IF	Nat Commun; Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.; Authors - Jing Yu
		Oncogene; Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.; Authors - A Motzik
human	WB	J Med Chem; A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide; Authors - Kellan T. Passow
human	IHC	Cell Cycle; The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.; Authors - Genovese Giannicola G
human,mouse	WB	Pharmacogenomics J; Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.; Authors - Jackson K J KJ
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J

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