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HINT1 Polyclonal antibody

HINT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10717-1-AP

Synonyms

HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1



Tested Applications

Positive WB detected inJurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

10717-1-AP targets HINT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HINT1 fusion protein Ag1142
Full Name histidine triad nucleotide binding protein 1
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC007090
Gene symbol HINT1
Gene ID (NCBI) 3094
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.

Protocols

Product Specific Protocols
WB protocol for HINT1 antibody 10717-1-APDownload protocol
IHC protocol for HINT1 antibody 10717-1-APDownload protocol
IP protocol for HINT1 antibody 10717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

The role of the Hint1 protein in the metabolism of phosphorothioate oligonucleotides drugs and prodrugs, and the release of H2S under cellular conditions.

Authors - Agnieszka Krakowiak
humanWB

Biochim Biophys Acta

Interactions of cellular histidine triad nucleotide binding protein 1 with nucleoside 5'-O-monophosphorothioates and their derivatives - Implication for desulfuration process in the cell.

Authors - Agnieszka Krakowiak
humanWB,IHC

Int J Oncol

Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.

Authors - Huang Huaying H
humanWB

Proteomics

Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.

Authors - Berglund Susanne R SR
human,mouseWB,IHC

Cancer Lett

Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.

Authors - Zhang Yu-Jing YJ
humanWB

J Biol Chem

Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.

Authors - Cen Bo B
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