Tested Applications
| Positive WB detected in | DU 145 cells, PC-3 cells, human brain tissue, A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human brain tissue, human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22231-1-AP targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17576 Product name: Recombinant human HIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 435-764 aa of BC110545 Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG Predict reactive species |
| Full Name | huntingtin interacting protein 1 |
| Calculated Molecular Weight | 1037 aa, 116 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | BC110545 |
| Gene Symbol | HIP1 |
| Gene ID (NCBI) | 3092 |
| RRID | AB_2879041 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O00291 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HIP1 antibody 22231-1-AP | Download protocol |
| IHC protocol for HIP1 antibody 22231-1-AP | Download protocol |
| IP protocol for HIP1 antibody 22231-1-AP | Download protocol |
| WB protocol for HIP1 antibody 22231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HIP1 Polyclonal antibody (Cat# 22231-1-AP) has 5 citations published in Ecotoxicology and Environmental Safety, Acta Biochimica et Biophysica Sinica, FASEB Journal, Life Science Alliance, and Oncology Letters. The associated research fields span reproductive ecotoxicology (sperm DNA damage), glioma tumorigenesis, endothelial angiogenesis and tissue repair, ubiquitin chain interactomics, and esophageal squamous cell carcinoma metastasis.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||
Acta Biochim Biophys Sin (Shanghai) Y-box protein-1 modulates circSPECC1 to promote glioma tumorigenesis via miR-615-5p/HIP1/AKT axis | ||
FASEB J MicroRNA-135a-3p regulates angiogenesis and tissue repair by targeting p38 signaling in endothelial cells.
| ||
Life Sci Alliance K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors | ||
Oncol Lett Abnormally high HIP1 expression is associated with metastatic behaviors and poor prognosis in ESCC. |
