HIP1-Specific Polyclonal antibody

HIP1-Specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19688-1-AP

Synonyms

HIP 1, HIP I, HIP1, ILWEQ



Tested Applications

Positive WB detected inHEK-293 cells, mouse colon tissue, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19688-1-AP targets HIP1-Specific in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name huntingtin interacting protein 1
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberNM_005338
Gene symbol HIP1
Gene ID (NCBI) 3092
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

Protocols

Product Specific Protocols
WB protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IHC protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IP protocol for HIP1-Specific antibody 19688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols