|Positive WB detected in||mouse brain tissue, mouse kidney tissue, human liver tissue, HeLa cells, mouse lung tissue, mouse heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16814-1-AP targets HIP1R in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HIP1R fusion protein Ag10353|
|Full Name||huntingtin interacting protein 1 related|
|Calculated molecular weight||615 aa, 70 kDa, 120 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC067085|
|Gene ID (NCBI)||9026|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Exp Biol Med (Maywood)
Proteomic analysis identifies NPTX1 and HIP1R as potential targets of histone deacetylase-3-mediated neurodegeneration.
J Clin Med
HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.
The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.
Nat Chem Biol
HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.
Nat Biomed Eng
Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.
Nat Cell Biol
Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.