HIP1R Polyclonal antibody

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• HIP1R Polyclonal antibody (16814-1-AP)

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• KD/KO Validated

HIP1R Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16814-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

• 

  WB analysis using 16814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 16814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 16814-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 16814-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16814-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 16814-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 16814-1-AP

  IP result of anti-HIP1R (IP:16814-1-AP, 4ug; Detection:16814-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16814-1-AP targets HIP1R in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10353

  Product name: Recombinant human HIP1R protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 266-615 aa of BC067085

  Sequence: RASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPSGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERVWPPQMQQHH

• Full Name: huntingtin interacting protein 1 related
• Calculated Molecular Weight: 615 aa, 70 kDa, 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC067085
• Gene Symbol: HIP1R
• Gene ID (NCBI): 9026
• RRID: AB_2117572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75146
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The HIP1R Polyclonal antibody (Cat# 16814-1-AP) has 13 citations. They appear in journals including Cell, Nature Biomedical Engineering, Nature Genetics, Nature Cell Biology, Nature Chemical Biology, Science Advances, Acta Pharmacologica Sinica, Bioengineering, RSC Chemical Biology, Experimental Biology and Medicine, Journal of Clinical Medicine, and Nature Communications. Research fields span cancer biology (pancreatic, melanoma, colorectal, lung), PD-L1/PD-1 immunotherapy, protein post-translational modifications (acetylation, palmitoylation, succinylation, methylation), neurodegeneration, and astrocyte biology.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa ; KD Validated
human	WB	Nat Biomed Eng; Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.; Authors - Han Yao
human	IP	Nat Genet; Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma; Authors - Long Liang
human	WB	Nat Cell Biol; Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.; Authors - Yang Gao ; KO Validated
human	WB,IP,IF	Nat Chem Biol; HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.; Authors - Huanbin Wang ; KD Validated
human	WB	Sci Adv; The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.; Authors - Riyad N H Seervai ; KD Validated