HIPK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HIPK2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 55408-1-AP

Synonyms

hHIPk2, HIPK2, PRO0593

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Validation Data Gallery

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  WB analysis of mouse kidney using 55408-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55408-1-AP (HIPK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

55408-1-AP targets HIPK2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: homeodomain interacting protein kinase 2
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 131 kDa, 101 kDa
• GenBank Accession Number: NM_022740
• Gene Symbol: HIPK2
• Gene ID (NCBI): 28996
• RRID: AB_2881323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIPK2 is a serine/threonine kinase participating in transcriptional regulation, growth control, and apoptosis. The kinase contributes to the regulation of remarkably diverse pathways such as p53 activation or Wnt signaling. There is very low levels of HIPK2 protein expression in several thyroid tumors and the combination of HIPK2 and Gal-3,would be helpful in ameliorating the preoperative recognition of thyroid cancer. HIPK2 may represent a new tumour suppressor gene for these types of cancers and may constitute a new potential promising diagnostic marker and therapeutic target.(PMID:21698151).

Publications

Species	Application	Title
mouse	IF	EBioMedicine; Inhibition of HIPK2 protects stress-induced pathological cardiac remodeling; Authors - Qiulian Zhou
mouse	WB	Chem Biol Interact; HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.; Authors - Xiaoyan Dang ; KD Validated
human	IHC	Microvasc Res; MiR-221-3p targets HIPK2 to promote diabetic wound healing.; Authors - Qiqi Yu ; KD Validated
human	WB,IHC	Cell Prolif; Homeodomain-interacting protein kinase 2 suppresses proliferation and aerobic glycolysis via ERK/cMyc axis in pancreatic cancer.; Authors - Yi Qin
human	WB	Int J Biol Macromol; Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.; Authors - Hui-Ying Hu
human,mouse	CoIP,WB	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu