HIRIP3 Polyclonal antibody

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HIRIP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14992-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

HIRA-interacting protein 3

• 

  WB analysis of HeLa using 14992-1-AP

  WB result of HIRIP3 antibody (14992-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 14992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human skeletal muscle using 14992-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 14992-1-AP

  IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, human heart tissue, human skeletal muscle tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tissue, human placenta tissue, human appendicitis tissue, human stomach cancer tissue, human tonsillitis tissue, human kidney tissue, human testis tissue, human heart tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6978

  Product name: Recombinant human HIRIP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 207-556 aa of BC000588

  Sequence: TAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN

• Full Name: HIRA interacting protein 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 69-90 kDa
• GenBank Accession Number: BC000588
• Gene Symbol: HIRIP3
• Gene ID (NCBI): 8479
• RRID: AB_2117876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BW71
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

Publications

What published studies show

The HIRIP3 Polyclonal Antibody (Cat# 14992-1-AP) has 1 total citation. The cited publication appeared in Frontiers in Cellular Neuroscience (IF 4.4, 2025), using the antibody for Western blot in mouse samples. The research field is Alzheimer's disease, specifically an integrative bioinformatics and machine learning study identifying iron metabolism genes (MAP4, GPT, and HIRIP3) as diagnostic biomarkers and therapeutic targets in Alzheimer's disease.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Front Cell Neurosci; Integrative bioinformatics and machine learning identify iron metabolism genes MAP4, GPT, and HIRIP3 as diagnostic biomarkers and therapeutic targets in Alzheimer's disease; Authors - Xiaoqiong An