Filter: AllWBIPIHC

WB analysis of HeLa using 14992-1-AP
WB result of HIRIP3 antibody (14992-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HeLa cells.
WB analysis of human heart using 14992-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 14992-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 14992-1-AP
IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.
IHC staining of human ovary using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach cancer using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 14992-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

HIRIP3 Polyclonal antibody

HIRIP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14992-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

HIRA-interacting protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, human skeletal muscle tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human stomach cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6978 Product name: Recombinant human HIRIP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-556 aa of BC000588 Sequence: TAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN Predict reactive species
Full Name	HIRA interacting protein 3
Calculated Molecular Weight	62 kDa
Observed Molecular Weight	69-90 kDa
GenBank Accession Number	BC000588
Gene Symbol	HIRIP3
Gene ID (NCBI)	8479
RRID	AB_2117876
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BW71
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

Protocols

Product Specific Protocols
IHC protocol for HIRIP3 antibody 14992-1-AP	Download protocol
IP protocol for HIRIP3 antibody 14992-1-AP	Download protocol
WB protocol for HIRIP3 antibody 14992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Front Cell Neurosci Integrative bioinformatics and machine learning identify iron metabolism genes MAP4, GPT, and HIRIP3 as diagnostic biomarkers and therapeutic targets in Alzheimer's disease Authors - Xiaoqiong An View Article