HIST1H2AC Polyclonal antibody

HIST1H2AC Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15953-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

H2A, H2AC6, H2A/l, H2A-clustered histone 6, H2AFL

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse liver using 15953-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat liver using 15953-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 15953-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 15953-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse lung using 15953-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat lung using 15953-1-AP
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat stomach using 15953-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 15953-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse liver tissue, rat liver tissue
Positive IHC detected in	human skin cancer tissue, rat stomach tissue, human stomach cancer tissue, rat lung tissue, mouse lung tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15953-1-AP targets HIST1H2AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8663 Product name: Recombinant human HIST1H2AC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-130 aa of BC017379 Sequence: MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK Predict reactive species
Full Name	histone cluster 1, H2ac
Calculated Molecular Weight	130 aa, 14 kDa
Observed Molecular Weight	17 kDa
GenBank Accession Number	BC017379
Gene Symbol	HIST1H2AC
Gene ID (NCBI)	8334
RRID	AB_2878199
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q93077
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HIST1H2AC antibody 15953-1-AP	Download protocol
WB protocol for HIST1H2AC antibody 15953-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HIST1H2AC polyclonal antibody (Cat# 15953-1-AP) has 1 total citation. It appears in Biology (Basel), in a study on identifying NRAS downstream genes using CRISPR activation screening. The research field is cancer genetics and gene regulatory network analysis, with the antibody applied in Western blot for human samples.