Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87007-1-RR (Acetyl-Histone H3 (Lys122) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.
Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87007-1-RR (Acetyl-Histone H3 (Lys122) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.
Dot Blot experiment of / using 87007-1-RR
Dot blot analysis was used to confirm the specificity of 87007-1-RR Acetyl-Histone H3 (Lys122) antibody antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
Dot blot analysis was used to confirm the specificity of 87007-1-RR Acetyl-Histone H3 (Lys122) antibody antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
ChIP experiment of HeLa using 87007-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys122) (87007-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys122) (87007-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87007-1-RR (Acetyl-Histone H3 (Lys122) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.
DOT BLOT Figures
Dot Blot experiment of / using 87007-1-RR
Dot blot analysis was used to confirm the specificity of 87007-1-RR Acetyl-Histone H3 (Lys122) antibody antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
CHIP-QPCR Figures
ChIP experiment of HeLa using 87007-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys122) (87007-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Acetyl-Histone H3 (Lys122) Recombinant antibody
Catalog Number
87007-1-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 DOT BLOT : 1:10-1:100 CHIP-QPCR : 1:10-1:100
Applications
WB, Dot Blot, ELISA, ChIP-qPCR
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
