Di-Methyl-Histone H4 (Arg3) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 82859-1-RR (same clone as 82859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82859-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 82859-1-RR (same clone as 82859-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82859-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of peptide using 82859-1-RR (same clone as 82859-1-PBS)

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82859-1-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of peptide using 82859-1-RR (same clone as 82859-1-PBS)

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82859-1-PBS in a different storage buffer formulation.

Di-Methyl-Histone H4 (Arg3) Uni-rAb^® Recombinant Antibody for WB, Dot Blot, Indirect ELISA

Cat No. 82859-1-PBS

Clone No.1E21

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, Indirect ELISA

Synonyms

Di Methyl Histone H4, Di-Methyl Histone H4 (Arg3), Di-Methyl Histone H4 Arg3, Di-Methyl Histone-H4 Arg3, Di-Methyl-Histone H4

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Product Information

82859-1-PBS targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H4a
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC069654
• Gene Symbol: HIST1H4A
• Gene ID (NCBI): 8359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62805
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).