Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15857-1-AP

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q



Tested Applications

Positive WB detected inHEK-293 cells, mouse pancreas tissue, Jurkat cells, rat kidney tissue, HeLa cells, U2OS cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inN/A

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15857-1-AP targets Histone H2B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag7811
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight14-17 kDa
GenBank accession numberBC005827
Gene symbol HIST2H2BE
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 15857-1-APDownload protocol
IHC protocol for Histone H2B antibody 15857-1-APDownload protocol
IP protocol for Histone H2B antibody 15857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.

Authors - Laura Monteonofrio
humanIF

Biol Open

In human sperm nucleus nucleosomes form spatially restricted domains consistent with programmed nucleosome positioning.

Authors - Mei-Zi Zhang

Int J Oncol

Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma.

Authors - Guang-Lin Song
mouseWB

Cell Cycle

Function of RAD6B and RNF8 in spermatogenesis.

Authors - Yingli Guo
mouseWB

Front Cell Neurosci

RAD6B Plays a Critical Role in Neuronal DNA Damage Response to Resist Neurodegeneration.

Authors - Zhao Guo
humanWB

J Biol Chem

Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2.

Authors - Whitcomb Sarah J SJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gunjita (Verified Customer) (01-06-2020)

N/A

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hek293
FH

Aurelie (Verified Customer) (11-19-2018)

strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: mouse cortex