Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse pancreas tissue, Jurkat cells, rat kidney tissue, HeLa cells, U2OS cells, mouse kidney tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human pancreas tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||N/A|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15857-1-AP targets Histone H2B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone H2B fusion protein Ag7811|
|Full Name||histone cluster 2, H2be|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14-17 kDa|
|GenBank accession number||BC005827|
|Gene ID (NCBI)||8349|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
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Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues
HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.
Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.
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Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.
USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gunjita (Verified Customer) (01-06-2020)
Aurelie (Verified Customer) (11-19-2018)
strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)