Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15857-1-AP

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q



Tested Applications

Positive WB detected inmouse pancreas tissue, rat kidney tissue, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15857-1-AP targets Histone H2B in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag7811
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
GenBank accession numberBC005827
Gene symbol HIST2H2BE
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 15857-1-APDownload protocol
IHC protocol for Histone H2B antibody 15857-1-APDownload protocol
IP protocol for Histone H2B antibody 15857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.

Authors - Laura Monteonofrio
humanIF

Biol Open

In human sperm nucleus nucleosomes form spatially restricted domains consistent with programmed nucleosome positioning.

Authors - Mei-Zi Zhang

Int J Oncol

Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma.

Authors - Guang-Lin Song
mouseWB

Cell Cycle

Function of RAD6B and RNF8 in spermatogenesis.

Authors - Yingli Guo
mouseWB

Front Cell Neurosci

RAD6B Plays a Critical Role in Neuronal DNA Damage Response to Resist Neurodegeneration.

Authors - Zhao Guo
humanWB

J Biol Chem

Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2.

Authors - Whitcomb Sarah J SJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gunjita (Verified Customer) (01-06-2020)

N/A

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hek293
FH

Aurelie (Verified Customer) (11-19-2018)

strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: mouse cortex