|Positive WB detected in||mouse pancreas tissue, rat kidney tissue, mouse kidney tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human pancreas tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15857-1-AP targets Histone H2B in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone H2B fusion protein Ag7811|
|Full Name||histone cluster 2, H2be|
|Calculated molecular weight||14 kDa|
|GenBank accession number||BC005827|
|Gene ID (NCBI)||8349|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.
In human sperm nucleus nucleosomes form spatially restricted domains consistent with programmed nucleosome positioning.
Int J Oncol
Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma.
Function of RAD6B and RNF8 in spermatogenesis.
Front Cell Neurosci
RAD6B Plays a Critical Role in Neuronal DNA Damage Response to Resist Neurodegeneration.
J Biol Chem
Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gunjita (Verified Customer) (01-06-2020)
Aurelie (Verified Customer) (11-19-2018)
strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)