Histone H2A type 3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Histone H2A type 3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10445-1-AP

Synonyms

HIST3H2A, histone, histone cluster 3, H2a, Histone H2A, Histone H2A type 3

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Validation Data Gallery

• 

  WB analysis of human brain using 10445-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (Histone H2A type 3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10445-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (Histone H2A type 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10445-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (HIST3H2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10445-1-AP targets Histone H2A type 3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H2A type 3 fusion protein Ag0374
• Full Name: histone cluster 3, H2a
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC001193
• Gene Symbol: Histone H2A type 3
• Gene ID (NCBI): 92815
• RRID: AB_2116701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone H2A type 3, it is expected to be located in the nucleus, and the protein is enriched in the brain tissue. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. The molecular weight of Histone H2A type 3 is 14 kDa.

Publications

Species	Application	Title
human,mouse	WB,IP,IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su ; KO Validated
mouse	WB	Nucleic Acids Res; Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder.; Authors - Tianjin Shen
mouse	IHC	EMBO Rep; MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors.; Authors - Hongyan Ma
human	WB	BMC Cancer; HIST3H2A promotes the progression of prostate cancer through inhibiting cell necroptosis; Authors - Lihong Yang