HIV-1-P24 Polyclonal antibody

HIV-1-P24 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Recombinant protein

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30168-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of Recombinant protein using 30168-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 30168-1-AP (HIV-1-P24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30168-1-AP targets HIV-1-P24 in WB, ELISA applications and shows reactivity with Recombinant protein samples.

• Tested Reactivity: Recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIV-1-P24 fusion protein Ag32891
• Full Name: Gag polyprotein
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: NP_057850
• Gene Symbol: gag
• Gene ID (NCBI): 155030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HIV virus is composed of core RNA, capsid, and the outermost envelope. Among them, the capsid is only composed of P24 protein, which is the product of structural gene GAG. GAG encodes a 55kda GAG protein P55, which is cleaved into capsid P24 by protease H. P24 surrounds the core genomic RNA of the HIV virus particles, forming a firm conical nucleocapsid complex. In addition, due to the high conservativeness of the GAG gene sequence, the P24 protein amino acid sequence is also highly conservative in different strains of HIV, so it can be used as a detection of antigen in HIV infection.