HJURP Monoclonal antibody

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  WB analysis using 68995-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68995-1-Ig (HJURP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 68995-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HJURP antibody (68995-1-Ig, Clone: 1F8C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

HJURP Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68995-1-Ig

Clone No.1F8C5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

1F8C5, FAKTS, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1

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Tested Applications

• Positive WB detected in: PANC-1 cells, SW 1990 cells, Saos2 cells, U2OS cells, A549 cells, HeLa cells, MDA-MB-468 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68995-1-Ig targets HJURP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HJURP fusion protein Ag34571
• Full Name: Holliday junction recognition protein
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC001940
• Gene Symbol: HJURP
• Gene ID (NCBI): 55355
• RRID: AB_3670458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8NCD3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)