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  • KD/KO Validated

Hexokinase 2 Polyclonal antibody

Hexokinase 2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 22029-1-AP


Hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase

Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse skeletal muscle tissue, Jurkat cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22029-1-AP is Hexokinase 2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC021116
Gene symbol HK2
Gene ID (NCBI) 3099
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)


Product Specific Protocols
WB protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IHC protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IP protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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