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  • KD/KO Validated

Hexokinase 2 Antibody

Hexokinase 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22029-1-AP

Synonyms

Hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase



Tested Applications

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, mouse skeletal muscle tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:600
Sample-dependent, check data in validation data gallery

Product Information

The 22029-1-AP immunogen is Fusion Protein Hexokinase 2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession number BC021116
Gene symbol HK2
Gene ID (NCBI) 3099
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IHC protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IP protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

Oxidized Vitamin C (DHA) Overcomes Resistance to EGFR-targeted Therapy of Lung Cancer through Disturbing Energy Homeostasis.

Authors - Mingtong Ye
humanIHC

Contrast Media Mol Imaging

Fructose 1,6-Bisphosphatase 1 Expression Reduces 18F-FDG Uptake in Clear Cell Renal Cell Carcinoma.

Authors - Ruohua Chen
humanWB

Cancer Res

Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer.

Authors - Dalong Cao
humanWB

Mol Cancer

Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer.

Authors - Jianan Chen
  • KD Validated
humanWB

Cell Commun Signal

Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells.

Authors - Feixia Wang
humanWB

Cancer Lett

UHRF1 promotes aerobic glycolysis and proliferation via suppression of SIRT4 in pancreatic cancer.

Authors - Qiangsheng Hu