Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25874-1-AP targets HKDC1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23045 Product name: Recombinant human HKDC1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-210 aa of BC110504 Sequence: MFAVHLMAFYFSKLKEDQIKKVDRFLYHMRLSDDTLLDIMRRFRAEMEKGLAKDTNPTAAVKMLPTFVRAIPDGSENGEFLSLDLGGSKFRVLKVQVAEEGKRHVQMESQFYPTPNEIIRGNGTELFEYVADCLADFMKTKDLKHKKLPLGLTFSFPCRQTKLEEGVLLSWTKKFKARGVQDTDVVSRLTKAMRRHKDMDVDILALVNDT Predict reactive species |
| Full Name | hexokinase domain containing 1 |
| Calculated Molecular Weight | 917 aa, 103 kDa |
| Observed Molecular Weight | 102 kDa, 82-90 kDa |
| GenBank Accession Number | BC110504 |
| Gene Symbol | HKDC1 |
| Gene ID (NCBI) | 80201 |
| RRID | AB_2880279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2TB90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HKDC1 antibody 25874-1-AP | Download protocol |
| WB protocol for HKDC1 antibody 25874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HKDC1 antibody (Cat# 25874-1-AP) has 14 citations spanning journals including Cell Death & Disease, Cell Reports, Communications Biology, iScience, FASEB Journal, eLife, International Journal of Molecular Sciences, and others. Research fields cover hepatocellular carcinoma, gastric cancer, ovarian cancer, pancreatic cancer, liver cirrhosis, colorectal cancer, polycystic ovary syndrome, mitochondrial respiration, lipid metabolism, glycolysis, and immune microenvironment studies, primarily using WB and IHC applications in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis HNF4α-HKDC1 axis orchestrates a metabolic rewiring to promote migration and metastasis in advanced gastric cancer. | ||
Cell Rep Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3
| ||
Commun Biol HKDC1 promotes ovarian cancer progression through boosting lipid metabolism and immune escape by stabilizing G6PC/G6PC2 | ||
Int J Mol Sci Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer | ||
Biochim Biophys Acta Mol Basis Dis HKDC1 promotes the H3K18 lactylation of the promoter of ORMDL3 to induce the activation of hepatic stellate cells in liver cirrhosis. | ||
Virol Sin Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection. |
