Tested Applications
| Positive WB detected in | AsPC-1 cells, Caki-1 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87988-1-RR targets HKDC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag23045 Product name: Recombinant human HKDC1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-210 aa of BC110504 Sequence: MFAVHLMAFYFSKLKEDQIKKVDRFLYHMRLSDDTLLDIMRRFRAEMEKGLAKDTNPTAAVKMLPTFVRAIPDGSENGEFLSLDLGGSKFRVLKVQVAEEGKRHVQMESQFYPTPNEIIRGNGTELFEYVADCLADFMKTKDLKHKKLPLGLTFSFPCRQTKLEEGVLLSWTKKFKARGVQDTDVVSRLTKAMRRHKDMDVDILALVNDT Predict reactive species |
| Full Name | hexokinase domain containing 1 |
| Calculated Molecular Weight | 917 aa, 103 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC110504 |
| Gene Symbol | HKDC1 |
| Gene ID (NCBI) | 80201 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q2TB90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HKDC1 (Hexokinase Domain Containing 1), encodes a 917-amino-acid protein with a molecular weight of approximately 102.5 kDa (102,545 Da). The protein is widely expressed, with higher expression in tissues such as the duodenum, small intestine, kidney, and colon, and primarily localizes to the cytoplasm and mitochondria. HKDC1 functions as a hexokinase that catalyzes the phosphorylation of hexose sugars, albeit at very low levels compared to other hexokinases. It plays a role in glucose metabolism and homeostasis, and is required to maintain whole-body glucose homeostasis during pregnancy. Additionally, HKDC1 is involved in coordinating mitochondrial and lysosomal homeostasis, which is critical for preventing cellular senescence.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HKDC1 antibody 87988-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
