HLA-A Polyclonal antibody

HLA-A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55383-1-AP

Synonyms

HLA A, HLAA, HLA-A, MHC class I antigen A*3

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Validation Data Gallery

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  WB analysis using 55383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55383-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, A431 cells
• Positive IHC detected in: human kidney tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

55383-1-AP targets HLA-A in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: major histocompatibility complex, class I, A
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41-44 kDa
• GenBank Accession Number: NM_002116
• Gene Symbol: HLA-A
• Gene ID (NCBI): 3105
• RRID: AB_10888639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	IF	J Virol; Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef.; Authors - Lucas A Tavares