HLA-DRA Polyclonal antibody

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• HLA-DRA Polyclonal antibody (17221-1-AP)

• KD/KO Validated

HLA-DRA Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 17221-1-AP Copied!

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

HLA DRA, HLA DRA1, HLA DRB, HLA-DR, HLA-DRA1

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  WB analysis using 17221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Daudi using 17221-1-AP

  WB result of HLA-DRA antibody (17221-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-DRA transfected Daudi cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat spleen using 17221-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Raji using 17221-1-AP

  IP result of anti-HLA-DRA (IP:17221-1-AP, 4ug; Detection:17221-1-AP 1:8000) with Raji cells lysate 1920 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 17221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA-DRA antibody (17221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 17221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA-DRA antibody (17221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Raji using 17221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (17221-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Daudi cells, rat spleen tissue, Raji cells, Ramos cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human appendicitis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17221-1-AP targets HLA-DRA in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11044

  Product name: Recombinant human HLA-DRA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-218 aa of BC032350

  Sequence: WAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENV

• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• RRID: AB_10639510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01903
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-HLA-DR antibody (Cat# 17221-1-AP) has 18 citations across journals including Nature Communications, Journal for Immunotherapy of Cancer, International Journal of Biological Sciences, Molecular Medicine, Frontiers in Immunology, BMC Cancer, European Journal of Immunology, and Aging Cell. Research fields span cancer immunology, tumor microenvironment characterization, HLA class II expression in immunotherapy response, single-cell/spatial transcriptomics, immune regulation, and reproductive immunology, with applications in IF, IHC, WB, and ELISA.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
human	IF	Int J Biol Sci; Single-cell and spatial transcriptomic analysis reveal cellular heterogeneity and cancer cell-intrinsic major histocompatibility complex II expression in urothelial carcinoma.; Authors - Shengwei Xiong
human	IHC	J Immunother Cancer; Dual regulation of CXCR6(+)CD8(+) T cells modulates cytotoxic and exhaustion-associated programs during prostate cancer progression.; Authors - Zhang Xingming
rat	IHC	Mol Med; Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production.; Authors - Mian Zhou
human	IHC	Mol Med; Crosstalk between cancer-associated fibroblasts and non-neuroendocrine tumor cells in small cell lung cancer involves in glycolysis and antigen-presenting features; Authors - Yuanhua Lu
rat	IF	Aging Cell; Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.; Authors - Zhi-Gang Wang