HLA-DRA Polyclonal antibody

HLA-DRA Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17221-1-AP

Synonyms

HLA DRA, HLA DRA1, HLA-DR, HLA-DRA, MHC class II antigen DRA



Tested Applications

Positive WB detected inDaudi cells, rat spleen tissue, Raji cells, Ramos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17221-1-AP targets HLA-DRA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRA fusion protein Ag11044
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 32-34 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
RRIDAB_10639510
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLA-DRA antibody 17221-1-APDownload protocol
IHC protocol for HLA-DRA antibody 17221-1-APDownload protocol
IP protocol for HLA-DRA antibody 17221-1-APDownload protocol
FC protocol for HLA-DRA antibody 17221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Authors - Zhaohui Chen
ratIF

Aging Cell

Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.

Authors - Zhi-Gang Wang
humanWB

Front Immunol

An Immunological Perspective: What Happened to Pregnant Women After Recovering From COVID-19?

Authors - Sijia Zhao
humanIF

Front Immunol

Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment.

Authors - Pin-Yuan Chen
ratIHC

Mol Med

Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production.

Authors - Mian Zhou
humanIHC

BMC Cancer

HLA class II molecule HLA-DRA identifies immuno-hot tumors and predicts the therapeutic response to anti-PD-1 immunotherapy in NSCLC.

Authors - Jie Mei