Validation Data Gallery
|Positive WB detected in
|Daudi cells, rat spleen tissue, Raji cells, Ramos cells
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, human kidney tissue, human tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17221-1-AP targets HLA-DRA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.
|Host / Isotype
|Rabbit / IgG
|HLA-DRA fusion protein Ag11044
|major histocompatibility complex, class II, DR alpha
|Calculated molecular weight
|254 aa, 29 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
|Product Specific Protocols
|WB protocol for HLA-DRA antibody 17221-1-AP
|IHC protocol for HLA-DRA antibody 17221-1-AP
|IP protocol for HLA-DRA antibody 17221-1-AP
|FC protocol for HLA-DRA antibody 17221-1-AP
|Click here to view our Standard Protocols
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