HLA-DRB4 Recombinant monoclonal antibody

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  WB analysis using 83103-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 83103-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 83103-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83103-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

HLA-DRB4 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83103-1-RR

Clone No.230352F10

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells, pig spleen tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83103-1-RR targets HLA-DRB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8492

  Product name: Recombinant human HLA-DRB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-266 aa of BC005312

  Sequence: MVCLKLPGGSCMAALTVTLTVLSSPLALAGDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSKMLSGVGGFVLGLLFLGTGLFIYFRNQKGHSGLQPTGLLS

• Full Name: major histocompatibility complex, class II, DR beta 4
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC005312
• Gene Symbol: HLA-DRB4
• Gene ID (NCBI): 3126
• RRID: AB_3670816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13762
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.