Tested Applications
| Positive WB detected in | Daudi cells, HeLa cells, HepG2 cells, HL-60 cells, Raji cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, mouse lung tissue, mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21702-1-AP targets HLA-DRB5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16307 Product name: Recombinant human HLA-DRB5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC009234 Sequence: MVCLKLPGGSYMAKLTVTLMVLSSPLALAGDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSKMLSGVGGFVLGLLFLGAGLFIYFKNQKGHSGLHPTGLVS Predict reactive species |
| Full Name | major histocompatibility complex, class II, DR beta 5 |
| Calculated Molecular Weight | 266 aa, 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC009234 |
| Gene Symbol | HLA-DRB5 |
| Gene ID (NCBI) | 3127 |
| RRID | AB_2878905 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q30154 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-DRB5 antibody 21702-1-AP | Download protocol |
| IHC protocol for HLA-DRB5 antibody 21702-1-AP | Download protocol |
| WB protocol for HLA-DRB5 antibody 21702-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HLA-DRB5 Polyclonal antibody (Cat# 21702-1-AP) has 1 citation. It appears in Open Medicine (Warsaw), published in 2024. The cited study investigates how HLA-DRB5 promotes immune thrombocytopenia by activating CD8+ T cells, placing the research in the fields of immunology and autoimmune hematological disorders. The antibody was applied in Western blot (WB) in this publication.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
The single review rates the antibody 4 out of 5 stars. At a 1:500 dilution, the user reports strong performance in immunohistochemistry and immunofluorescence on both tissue (brain) and cells (microglia). However, the antibody showed less satisfactory results in Western blot compared to the other two applications. Overall, it is well-suited for IHC and IF but may require optimization for WB use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sofia (Verified Customer) (02-03-2025) | Antibody works fine in IHC and IF, in tissue and cells. For Western blot doesn't work as well as in the other techniques.
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