HLTF Polyclonal antibody

• Featured Product
• KD/KO Validated

HLTF Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 14286-1-AP

Synonyms

HLTF1, HIP116A, HIP116, Helicase-like transcription factor, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis of HeLa using 14286-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14286-1-AP

  IP result of anti-HLTF (IP:14286-1-AP, 4ug; Detection:14286-1-AP 1:500) with HeLa cells lysate 2400ug.

• 

  IF Staining of HeLa using 14286-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

14286-1-AP targets HLTF in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLTF fusion protein Ag5596
• Full Name: helicase-like transcription factor
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC044659
• Gene Symbol: HLTF
• Gene ID (NCBI): 6596
• RRID: AB_2279646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Genes Dev; Break-induced replication promotes fragile telomere formation.; Authors - Zhe Yang
human	WB	Elife; Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.; Authors - Kerrie-Ann McMahon
human	WB	Front Immunol; Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.; Authors - Wenying Gao
human	WB,IHC	Int J Oncol; HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.; Authors - Li Liu ; KD Validated
human	CoIP	Oncogenesis; HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway; Authors - Yanan Xu ; KD Validated
mouse	WB	Cell Rep; A rewiring of DNA replication mediated by MRE11 exonuclease underlies primed-to-naive cell de-differentiation; Authors - Patricia Ubieto-Capella ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (07-12-2024)

Work perfectly not only with overexpressed protein, but with endogenous ones as well.

• Applications: Western Blot, Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

HLTF Antibody Western Blot,Immunofluorescence,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:14286-1-AP)