HLX Polyclonal antibody

• Featured Product
• KD/KO Validated

HLX Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14336-1-AP

Synonyms

Homeobox protein HB24, HLX1, HB24, H2.0-like homeobox protein, H2.0 like homeobox protein

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Validation Data Gallery

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  WB analysis of human placenta using 14336-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14336-1-AP (HLX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 14336-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 14336-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 14336-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14336-1-AP (HLX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14336-1-AP targets HLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLX fusion protein Ag5627
• Full Name: H2.0-like homeobox
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC033808
• Gene Symbol: HLX
• Gene ID (NCBI): 3142
• RRID: AB_2232971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In immune system, Hlx is a Th1-special transcription factor that interacts specifically with T-bet, and synergistically they promote IFN-γ expression when co-expressed. It can down-regulate the IL-4 receptor expression in naive CD4+ T cells [PMID:14688316]. Over expression of Hlx contributes ot the aberrant expression of IFN-γ in normal CD4+ T cells during the differentiation under Th2-polarizing conditions. In addition, Hlx is a negative regulator of IFN-γ production in NK cells and its inhibitory function is achieved at least in part through the proteasomal degradation of STAT4 [PMID:16424184].

Publications

Species	Application	Title
human	WB,IHC	Tumour Biol; H2.0-like homeobox 1 acts as a tumor suppressor in hepatocellular carcinoma.; Authors - Ting Liu ; KD Validated