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  • KD/KO Validated

HMBOX1 Polyclonal antibody

HMBOX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16123-1-AP

Synonyms

HMBOX1, HNF1LA, homeobox containing 1, Homeobox containing protein 1, PBHNF



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16123-1-AP targets HMBOX1 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMBOX1 fusion protein Ag9081
Full Name homeobox containing 1
Calculated Molecular Weight 420 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC009259
Gene Symbol HMBOX1
Gene ID (NCBI) 79618
RRIDAB_10794445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HMBOX1 antibody 16123-1-APDownload protocol
IP protocol for HMBOX1 antibody 16123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ChIP

Mol Ther

Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.

Authors - Chenfei Zhou
  • KD Validated
human,ratWB

Front Cardiovasc Med

Exosome-Derived From Sepsis Patients' Blood Promoted Pyroptosis of Cardiomyocytes by Regulating miR-885-5p/HMBOX1.

Authors - Guo-Wei Tu

Oncol Rep

Knockdown of homeobox containing 1 increases the radiosensitivity of cervical cancer cells through telomere shortening.

Authors - Shuliang Zhou
humanWB

Exp Ther Med

HMBOX1 attenuates LPS-induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF-κB signaling pathway.

Authors - Minyuan Nie
humanWB,IHC

Cell Death Dis

WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m6A-dependent manner.

Authors - Shijie Chen
  • KD Validated
humanWB

J Cell Mol Med

5,2'-dibromo-2,4',5'-trihydroxydiphenylmethanone attenuates LPS-induced inflammation and ROS production in EA.hy926 cells via HMBOX1 induction.

Authors - Hong-Xia Yuan