HMCN1 Polyclonal antibody
HMCN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 18837-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skin tissue |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | hTERT-RPE1 cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
Product Information
18837-1-AP targets HMCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | hemicentin 1 |
Calculated Molecular Weight | 613 kDa, 600 kDa, 60 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | NM_031935 |
Gene Symbol | HMCN1 |
Gene ID (NCBI) | 83872 |
RRID | AB_2878563 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.
Protocols
Product Specific Protocols | |
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WB protocol for HMCN1 antibody 18837-1-AP | Download protocol |
IHC protocol for HMCN1 antibody 18837-1-AP | Download protocol |
IF protocol for HMCN1 antibody 18837-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Pharmacol Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1 | ||
Front Cell Dev Biol Hemicentin-1 is an essential extracellular matrix component during tooth root formation by promoting mesenchymal cells differentiation |