HMG20A Monoclonal antibody

HMG20A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10F12

Cat no : 66676-1-Ig

Synonyms

FLJ10739, high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1

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Validation Data Gallery

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  WB analysis of HeLa using 66676-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66676-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66676-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66676-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66676-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, NCCIT cell
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66676-1-Ig targets HMG20A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMG20A fusion protein Ag2718
• Full Name: high-mobility group 20A
• Calculated Molecular Weight: 347 aa, 40 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC021959
• Gene Symbol: HMG20A
• Gene ID (NCBI): 10363
• RRID: AB_2882030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation