HMG20B Monoclonal antibody, PBS Only

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  WB analysis using 67354-1-Ig (same clone as 67354-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

HMG20B Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67354-1-PBS

Clone No.4C2A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

4C2A9, BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35

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Product Information

67354-1-PBS targets HMG20B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6103

  Product name: Recombinant human HMG20B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 73-284 aa of BC002552

  Sequence: PVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYM

• Full Name: high-mobility group 20B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002552
• Gene Symbol: HMG20B
• Gene ID (NCBI): 10362
• RRID: AB_2882609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9P0W2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation (PMID:10449787). The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse (PMID:15240883). HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure (PMID:19997604). The predicted molecular weight of the HMG20B protein is 36 kDa, while the molecular weight detected in the literature is approximately 40 kDa by SDS-PAGE (PMID: 36171271).