Tested Applications
| Positive WB detected in | HepG2 cells, MKN-45 cells |
| Positive IHC detected in | human prostate cancer tissue, human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
29895-1-AP targets HMGA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31866 Product name: Recombinant human HMGA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-77 aa of BC071864 Sequence: SESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTT Predict reactive species |
| Full Name | high mobility group AT-hook 1 |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC071864 |
| Gene Symbol | HMGA1 |
| Gene ID (NCBI) | 3159 |
| RRID | AB_2923618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17096 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HMGA1 antibody 29895-1-AP | Download protocol |
| IHC protocol for HMGA1 antibody 29895-1-AP | Download protocol |
| WB protocol for HMGA1 antibody 29895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer | ||
Pathol Oncol Res Long non-coding RNA MYU promotes ovarian cancer cell proliferation by sponging miR-6827-5p and upregulating HMGA1 | ||
Chem Sci A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation | ||
EPMA J Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies | ||
J Thorac Dis Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients | ||
