Tested Applications
| Positive WB detected in | mouse testis tissue, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12787-1-AP targets HMGB4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3503 Product name: Recombinant human HMGB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC021180 Sequence: MGKEIQLKPKANVSSYVHFLLNYRNKFKEQQPSTYVGFKEFSRKCSEKWRSISKHEKAKYEALAKLDKARYQEEMMNYVGKRKKRRKRDPQAPRRPPSSFLLFCQDHYAQLKRENPNWSVVQVAKATGKMWSTATDLEKHPYEQRVALLRAKYFEELELYRKQCNARKKYRMSARNRCRGKRVRQS Predict reactive species |
| Full Name | high-mobility group box 4 |
| Calculated Molecular Weight | 186 aa, 22 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC021180 |
| Gene Symbol | HMGB4 |
| Gene ID (NCBI) | 127540 |
| RRID | AB_10638490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WW32 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
High mobility group protein B4 (HMGB4) is a new member in the family of HMGB proteins. Unlike other HMGB-proteins, it is a mammalian specific protein that has two HMGB-boxes but lacks the acidic tail. HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues (PMID: 18987332). It binds strongly to nuclear structures, regulates chromatin and mediates differentiation of neuronal cells (PMID: 27608812).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGB4 antibody 12787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HMGB4 Polyclonal antibody (Cat# 12787-1-AP) has 3 citations published in Cell Death & Disease, Journal for ImmunoTherapy of Cancer, and bioRxiv. Research fields span tumor biology (nasopharyngeal carcinoma metastasis and DLBCL progression), immune regulation (macrophage polarization), and gut microbiota–epithelial interactions (colonic acetyl-CoA metabolism and senescence).
| Species | Application | Title |
|---|---|---|
Cell Death Dis Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis. | ||
J Immunother Cancer Tumor-derived HMGB2 induces M2-like macrophage polarization via TRIM65-mediated NLRP3 degradation to promote DLBCL progression. | ||
bioRxiv Gut Microbiota-derived Acetate Safeguards the Colonic Epithelial Acetyl-CoA Reserve to Avert Colonic Senescence.
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