HMGCL Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84769-4-RR (same clone as 84769-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84769-4-RR (same clone as 84769-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84769-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

HMGCL Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84769-4-PBS

Clone No.242224F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

242224F9, EC:4.1.3.4, HL, HMG CoA lyase, HMG-CoA lyase

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Product Information

84769-4-PBS targets HMGCL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10426

  Product name: Recombinant human HMGCL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC010570

  Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL

• Full Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Calculated Molecular Weight: 325 aa, 34 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010570
• Gene Symbol: HMGCL
• Gene ID (NCBI): 3155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35914
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.