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  • KD/KO Validated

HMGCR Polyclonal antibody

HMGCR Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13533-1-AP

Synonyms

HMG CoA reductase, HMGCR



Tested Applications

Positive IHC detected inhuman liver cancer tissue, human small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13533-1-AP targets HMGCR in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGCR fusion protein Ag4444
Full Name 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Calculated molecular weight 888 aa, 95 kDa
GenBank accession numberBC033692
Gene symbol HMGCR
Gene ID (NCBI) 3156
RRIDAB_2877957
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGCR(3-hydroxy-3-methylglutaryl-coenzyme A reductase) is also named as HMG-CoA reductase and belongs to the HMG-CoA reductase family. This protein exists as a 97-kDa glycoprotein in the endoplasmic reticulum (ER) and is the rate-determining enzyme of the biosynthesis pathway(PMID:15830340).The ectopic expression of either full-length HMGCR or its novel splice variant promotes dysregulation of the MVA pathway. And the ectopic expression of cHMGCR-FL(60 kDa) and cHMGCR-D13(55 kDa) can be detected in HepG2 cells(PMID:20696928).This protein is also can exsit as a oligomer(PMID:20696928).

Protocols

Product Specific Protocols
IHC protocol for HMGCR antibody 13533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
humanIF

J Cell Biol

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Authors - Wan Yun Ho
  • KD Validated
humanIHC

Acta Pharmacol Sin

Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.

Authors - Hai-Yan Wang
  • KD Validated
human

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
human

PLoS Pathog

Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.

Authors - Liang Wei Wang
humanIHC

Mol Metab

LDLR inhibition promotes hepatocellular carcinoma proliferation and metastasis by elevating intracellular cholesterol synthesis through the MEK/ERK signaling pathway.

Authors - Ziye Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Malak (Verified Customer) (11-30-2020)

The antibody works very well in IHC staining especially at pH6 and with 1/100 dilution

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Hepatic tissue