Validation Data Gallery
|Positive IHC detected in
|human liver cancer tissue, human small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|IHC : 1:500-1:2000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13533-1-AP targets HMGCR in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|HMGCR fusion protein Ag4444
|3-hydroxy-3-methylglutaryl-Coenzyme A reductase
|Calculated molecular weight
|888 aa, 95 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
HMGCR(3-hydroxy-3-methylglutaryl-coenzyme A reductase) is also named as HMG-CoA reductase and belongs to the HMG-CoA reductase family. This protein exists as a 97-kDa glycoprotein in the endoplasmic reticulum (ER) and is the rate-determining enzyme of the biosynthesis pathway(PMID:15830340).The ectopic expression of either full-length HMGCR or its novel splice variant promotes dysregulation of the MVA pathway. And the ectopic expression of cHMGCR-FL(60 kDa) and cHMGCR-D13(55 kDa) can be detected in HepG2 cells(PMID:20696928).This protein is also can exsit as a oligomer(PMID:20696928).
Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.
J Cell Biol
TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.
Acta Pharmacol Sin
Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.
Cell Mol Gastroenterol Hepatol
A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.
Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.
LDLR inhibition promotes hepatocellular carcinoma proliferation and metastasis by elevating intracellular cholesterol synthesis through the MEK/ERK signaling pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Malak (Verified Customer) (11-30-2020)
The antibody works very well in IHC staining especially at pH6 and with 1/100 dilution