HMGN2 Polyclonal antibody

HMGN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10953-1-AP

Synonyms

Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2

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Validation Data Gallery

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  WB analysis of Jurkat using 10953-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10953-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10953-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10953-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10953-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10953-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGN2 antibody (10953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, HL-60 cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• ELISA: See 1 publications below

Product Information

10953-1-AP targets HMGN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGN2 fusion protein Ag1401
• Full Name: high-mobility group nucleosomal binding domain 2
• Calculated Molecular Weight: 9 aa, 3 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC014644
• Gene Symbol: HMGN2
• Gene ID (NCBI): 3151
• RRID: AB_2118482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance; Authors - Mengting Xu
human	ELISA	Mol Cancer; HMGN2, a new anti-tumor effector molecule of CD8+ T cells.; Authors - Lin Su
pig	WB	J Proteomics; DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase; Authors - Chen Zhou