HMGXB4 Polyclonal antibody, PBS Only

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• HMGXB4 Polyclonal antibody, PBS Only (24821-1-PBS)

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  WB analysis of HeLa using 24821-1-AP (same clone as 24821-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24821-1-AP (HMGXB4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24821-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 24821-1-AP (same clone as 24821-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24821-1-AP (HMGXB4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24821-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 24821-1-AP (same clone as 24821-1-PBS)

  IP result of anti-HMGXB4 (IP:24821-1-AP, 4ug; Detection:24821-1-AP 1:500) with HeLa cells lysate 2560ug. This data was developed using the same antibody clone with 24821-1-PBS in a different storage buffer formulation.

HMGXB4 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 24821-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

24821-1-PBS targets HMGXB4 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20424

  Product name: Recombinant human HMGXB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 145-492 aa of BC104754

  Sequence: SSSDAHSSPGPEGCGSDASQFAESHSANLDLSGLEPILVESDSSSGGELEAGELVIDDSYREIKKKKKSKKSKKKKDKEKHKEKRHSKSKRSLGLSAVPVGEVTVTSGPPPSIPYAGAAAPPLPLPGLHTDGHSEKKKKKEEKDKERERGEKPKKKNMSAYQVFCKEYRVTIVADHPGIDFGELSKKLAEVWKQLPEKDKLIWKQKAQYLQHKQNKAEATTVKRKASSSEGSMKVKASSVGVLSPQKKSPPTTMLLPASPAKAPETEPIDVAAHLQLLGESLSLIGHRLQETEGMVAVSGSLSVLLDSIICALGPLACLTTQLPELNGCPKQVLSNTLDNIAYIMPGL

• Full Name: HMG box domain containing 4
• Calculated Molecular Weight: 601 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC104754
• Gene Symbol: HMGXB4
• Gene ID (NCBI): 10042
• RRID: AB_2879742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGU5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HMGXB4 also termed as HMG box containing protein 4 or HMG2L1 is a 601 amino acid protein, which contains one HMG box DNA-binding domain. HMGXB4 negatively regulates Wnt/beta-catenin signaling during development. HMG-2L1 is expressed in the nucleus and may play a role in transcriptional regulation.