HMHA1 Polyclonal antibody

HMHA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14832-1-AP

Synonyms

HA 1, HLA HA1, HMHA1, KIAA0223, mHag HA 1

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Validation Data Gallery

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  WB analysis using 14832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14832-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14832-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 14832-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsil using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A375 cells, K-562 cells, mouse thymus tissue, HeLa cells, Jurkat cells
• Positive IHC detected in: human tonsil tissue, human spleen tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

14832-1-AP targets HMHA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMHA1 fusion protein Ag6636
• Full Name: histocompatibility (minor) HA-1
• Calculated Molecular Weight: 1136 aa, 125 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC065223
• Gene Symbol: HMHA1
• Gene ID (NCBI): 23526
• RRID: AB_10642145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Aging Dis; Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke; Authors - Hongyu Ma