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HNF1B Recombinant monoclonal antibody, PBS Only (84684-5-PBS)

HNF1B Recombinant monoclonal antibody, PBS Only

Name: HNF1B Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 84684-5-PBS-100UG

HNF1B Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84684-5-PBS

Clone No. 242120H8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllIF/ICCFC (Intra)Affinity

IF Staining of HepG2 using 84684-5-RR (same clone as 84684-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Caco-2 using 84684-5-RR (same clone as 84684-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Caco-2 using 84684-5-RR (same clone as 84684-5-PBS)
1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug HNF1B Recombinant antibody (84684-5-RR, Clone:242120H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug HNF1B Recombinant antibody (84684-5-RR, Clone:242120H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 84684-5-RR
Biolayer interferometry (BLl) kinetic assays of 84684-5-RR against Human HNF1B were performed. The affinity constant is 13.5 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

84684-5-PBS targets HNF1B in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3232 Product name: Recombinant human HNF1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC017714 Sequence: MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFR Predict reactive species
Full Name	HNF1 homeobox B
Calculated Molecular Weight	557 aa, 61 kDa
GenBank Accession Number	BC017714
Gene Symbol	HNF1B
Gene ID (NCBI)	6928
RRID	AB_3743663
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35680
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.