HNF1B Recombinant monoclonal antibody, PBS Only

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  IF Staining of HepG2 using 84684-5-RR (same clone as 84684-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 84684-5-RR (same clone as 84684-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 84684-5-RR (same clone as 84684-5-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug HNF1B Recombinant antibody (84684-5-RR, Clone:242120H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84684-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84684-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84684-5-RR against Human HNF1B were performed. The affinity constant is 13.5 nM.

HNF1B Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84684-5-PBS

Clone No.242120H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

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Product Information

84684-5-PBS targets HNF1B in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3232

  Product name: Recombinant human HNF1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC017714

  Sequence: MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFR

• Full Name: HNF1 homeobox B
• Calculated Molecular Weight: 557 aa, 61 kDa
• GenBank Accession Number: BC017714
• Gene Symbol: HNF1B
• Gene ID (NCBI): 6928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35680
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.