HNF4A Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HepG2 using 84316-3-RR (same clone as 84316-3-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84316-3-RR (HNF4A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84316-3-PBS in a different storage buffer formulation.

HNF4A Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84316-3-PBS

Clone No.241593C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

241593C4, Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7

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Product Information

84316-3-PBS targets HNF4A in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag24647

  Product name: Recombinant human HNF4A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC137539

  Sequence: MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ

• Full Name: hepatocyte nuclear factor 4, alpha
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC137539
• Gene Symbol: HNF4A
• Gene ID (NCBI): 3172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41235
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for treating liver fibrosis (PMID:34453962).