Tested Applications
| Positive WB detected in | HT-29 cells, human liver tissue, mouse liver tissue, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11874-1-AP targets HNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2462 Product name: Recombinant human HNMT protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-292 aa of BC020677 Sequence: MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGDENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA Predict reactive species |
| Full Name | histamine N-methyltransferase |
| Calculated Molecular Weight | 292 aa, 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC020677 |
| Gene Symbol | HNMT |
| Gene ID (NCBI) | 3176 |
| RRID | AB_2117048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50135 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNMT antibody 11874-1-AP | Download protocol |
| IHC protocol for HNMT antibody 11874-1-AP | Download protocol |
| WB protocol for HNMT antibody 11874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HNMT Polyclonal antibody (Cat# 11874-1-AP) has 3 total citations. These appear in Cell Stem Cell, Glia, and Current Medical Science. The associated research fields include glioblastoma stem cell biology and tumor angiogenesis, histamine clearance mechanisms in primary human astrocytes, and nasopharyngeal carcinoma progression involving the IFN/TXNIP/p53 signaling axis. Applications cited include Western blot and immunohistochemistry, all in human samples.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling | ||
Curr Med Sci HNMT Promotes the Occurrence and Progression of Nasopharyngeal Carcinoma by Inhibiting the IFN/TXNIP/p53 Axis |
