HNRNPA0 Polyclonal antibody
HNRNPA0 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 10848-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, fetal human brain tissue, mouse brain tissue, Jurkat cells, NIH/3T3 cells |
Positive IP detected in | mouse brain tissue |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
10848-1-AP targets HNRNPA0 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HNRNPA0 fusion protein Ag1312 |
Full Name | heterogeneous nuclear ribonucleoprotein A0 |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC019271 |
Gene Symbol | HNRNPA0 |
Gene ID (NCBI) | 10949 |
RRID | AB_2117066 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HNRNPA0 antibody 10848-1-AP | Download protocol |
IF protocol for HNRNPA0 antibody 10848-1-AP | Download protocol |
IP protocol for HNRNPA0 antibody 10848-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xiaochen (Verified Customer) (07-31-2023) | hnrnpa0 has many subtypes and thus got two bands, I think it is acceptable. Antibody quality is prety good.
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