Validation Data Gallery
|Positive WB detected in||A431 cells, A549 cells, Jurkat cells, Raji cells, HL-60 cells, HSC-T6 cells, HeLa cells, K-562 cells|
|Positive IHC detected in||human lymphoma tissue, human colon cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14813-1-AP targets HNRNPA2B1 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HNRNPA2B1 fusion protein Ag6563|
|Full Name||heterogeneous nuclear ribonucleoprotein A2/B1|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||36 kDa and 38 kDa|
|GenBank accession number||BC000506|
|Gene ID (NCBI)||3181|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HnRNPA2B1 in one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encode two protein, HNRNPA2 (38kd) and HNRNPB1 (36kd), by alternative splicing.
METTL3 regulates heterochromatin in mouse embryonic stem cells.
CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner
Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.
PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.
Integrative Analysis of NSCLC Identifies LINC01234 as an Oncogenic lncRNA that Interacts with HNRNPA2B1 and Regulates miR-106b Biogenesis.
Hum Mol Genet
Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.