HNRNPA2B1 Recombinant monoclonal antibody

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• HNRNPA2B1 Recombinant monoclonal antibody (86713-1-MR)

HNRNPA2B1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86713-1-MR

Clone No.V3H6F7F2

Host / Isotype

Mouse / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPB1, HNRPA2

• 

  WB analysis using 86713-1-MR

  Various lysates were subjected to SDS PAGE followed by western blot with 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 86713-1-MR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 86713-1-MR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:15000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 86713-1-MR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPA2B1 antibody (86713-1-MR, Clone: V3H6F7F2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 86713-1-MR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (86713-1-MR, Clone: V3H6F7F2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:7500-1:30000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86713-1-MR targets HNRNPA2B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6699

  Product name: Recombinant human HNRNPA2B1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-249 aa of BC000506

  Sequence: MEREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEDLEVAILEVAPVMEEEEEDMVVEDLDMATRVGATEVVMTTMEEEIMEVEITMILEIITSNLLTTVQ

• Full Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 34, 36 kDa
• GenBank Accession Number: BC000506
• Gene Symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• RRID: AB_3745072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22626
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.