Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14974-1-AP targets HNRNPF in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6885 Product name: Recombinant human HNRNPF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-415 aa of BC001432 Sequence: MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD Predict reactive species |
| Full Name | heterogeneous nuclear ribonucleoprotein F |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC001432 |
| Gene Symbol | HNRNPF |
| Gene ID (NCBI) | 3185 |
| RRID | AB_2263984 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPF antibody 14974-1-AP | Download protocol |
| IHC protocol for HNRNPF antibody 14974-1-AP | Download protocol |
| WB protocol for HNRNPF antibody 14974-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-HNRNPF antibody (Cat# 14974-1-AP) has 7 citations across journals including Free Radical Biology and Medicine, Cell Biosciences, Marine Drugs, BBA Molecular Basis of Disease, Virology Journal, Frontiers in Oncology, and bioRxiv. Research fields span hypoxic pulmonary hypertension and mitophagy, viral RNA splicing and replication, bladder and breast cancer, colorectal adenocarcinoma biomarkers, and epigenetic regulation of viral reactivation. Applications include WB, RIP, IF, IP, and IHC, all in human samples.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med LINC03047 promotes mitophagy by recruiting hnRNPF to enhance CTGF mRNA stability in hypoxic pulmonary hypertension. | ||
Cell Biosci PFV Tas protein recruits hnRNPF to promote the splicing of tas/bet pre-mRNA.
| ||
Mar Drugs An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells. | ||
Biochim Biophys Acta Mol Basis Dis HNRNPF-driven retention of exon 14 in HIF1A pre-mRNA promotes breast cancer metastasis. | ||
Virol J Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication.
| ||
Front Oncol The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma. |
Reviews
What customers say
The single review for this antibody (rated 4/5) describes its use in immunofluorescence on HeLa cells at a 1:1000 dilution, with overnight incubation at 4°C in 5% goat serum/PBST. The user reported a nuclear signal that was somewhat weak, along with some cytoplasmic staining. Overall, the antibody was considered functional for IF, though the signal intensity could be stronger.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (06-15-2025) | Incubation ON at 4C, in 5% goat serum/PBST. Gives nuclear signal but quite weak, some cytoplasmic staining also.
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