HNRNPF Polyclonal antibody

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  WB analysis of HeLa using 14974-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14974-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14974-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14974-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14974-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14974-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HNRNPF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14974-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Nucleolin-like protein mcs94-1, Nucleolin like protein mcs94 1, mcs94 1, HNRPF, hnRNP F

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14974-1-AP targets HNRNPF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6885

  Product name: Recombinant human HNRNPF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-415 aa of BC001432

  Sequence: MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD

• Full Name: heterogeneous nuclear ribonucleoprotein F
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC001432
• Gene Symbol: HNRNPF
• Gene ID (NCBI): 3185
• RRID: AB_2263984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mar Drugs; An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.; Authors - Su Ching-Chyuan CC
human	WB,IF,IP	Virol J; Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication.; Authors - Aiguo Zhang ; KD Validated
human	IHC	Front Oncol; The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.; Authors - Yixin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (06-15-2025)

Incubation ON at 4C, in 5% goat serum/PBST. Gives nuclear signal but quite weak, some cytoplasmic staining also.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa/human

HNRNPF Antibody Immunofluorescence validation (1:1000 dilution) in HeLa/human (Cat no:14974-1-AP)