HNRNPH1 Recombinant monoclonal antibody

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  WB analysis using 88017-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88017-1-RR (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 88017-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPH1 antibody (88017-1-RR, Clone: 260181B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 88017-1-RR

  Biolayer interferometry (BLI) kinetic assays of 88017-1-RR against Human HNRNPH1 were performed. The affinity constant is 0.905 nM.

HNRNPH1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88017-1-RR

Clone No.260181B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293T cells, 4T1 cells, NIH/3T3 cells, HSC-T6 cells, mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88017-1-RR targets HNRNPH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6506

  Product name: Recombinant human HNRNPH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 104-449 aa of BC001348

  Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

• Full Name: heterogeneous nuclear ribonucleoprotein H1 (H)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49-50 kDa
• GenBank Accession Number: BC001348
• Gene Symbol: HNRNPH1
• Gene ID (NCBI): 3187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31943
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].