HNRNPM Recombinant antibody

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  WB analysis using 85422-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85422-1-RR (HNRNPM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85422-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPM antibody (85422-1-RR, Clone: 242883C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HNRNPM Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85422-1-RR

Clone No.242883C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242883C3, hnRNP M, HNRNPM4, HNRPM, HNRPM4

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, COLO 320 cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85422-1-RR targets HNRNPM in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNRNPM fusion protein Ag25474
• Full Name: heterogeneous nuclear ribonucleoprotein M
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 73-77 kDa
• GenBank Accession Number: BC000138
• Gene Symbol: HNRNPM
• Gene ID (NCBI): 4670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52272
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPM, also named as NAGR1, is a 730 amino acid protein, which localizes in nucleus. HNRNPM as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. HNRNPM is Involved in splicing. HNRNPM acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. HNRNPM exists three isoforms MW range form 73 kDa and 77 kDa.