HNRNPR Polyclonal antibody

• Featured Product
• KD/KO Validated

HNRNPR Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15018-1-AP

Synonyms

hnRNP R, HNRNPR, HNRPR

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Validation Data Gallery

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  WB analysis of HepG2 using 15018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15018-1-AP (HNRNPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15018-1-AP (HNRNPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15018-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15018-1-AP (HNRNPR antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15018-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15018-1-AP (HNRNPR antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15018-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15018-1-AP (HNRNPR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

15018-1-AP targets HNRNPR in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPR fusion protein Ag7028
• Full Name: heterogeneous nuclear ribonucleoprotein R
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 65-82 kDa
• GenBank Accession Number: BC001449
• Gene Symbol: HNRNPR
• Gene ID (NCBI): 10236
• RRID: AB_2120506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

Publications

Species	Application	Title
human	WB	J Immunol; HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.; Authors - Adi Reches ; KD Validated
mouse	WB	Neurosci Lett; hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.; Authors - Akinsola Raphael Akinyemi
human	IHC,WB	Front Oncol; The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.; Authors - Yixin Li
	WB	iScience; Increased LDL receptor by SREBP2 or SREBP2-induced lncRNA LDLR-AS promotes triglyceride accumulation in fish; Authors - Xiufei Cao ; KD Validated
human	IHC	J Transl Med; High expression of HNRNPR in ESCA combined with 18F-FDG PET/CT metabolic parameters are novel biomarkers for preoperative diagnosis of ESCA; Authors - Xiao-Yu Liu
human	WB	Eur J Pharm Sci; Novel facile thermosensitive hydrogel as sustained and controllable gene release vehicle for breast cancer treatment.; Authors - Dan Zhao