HNRNPR Recombinant antibody, PBS Only

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  WB analysis using 85078-5-RR (same clone as 85078-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85078-5-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85078-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85078-5-RR (same clone as 85078-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85078-5-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85078-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85078-5-RR (same clone as 85078-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPR antibody (85078-5-RR, Clone: 242734A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85078-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85078-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85078-5-RR against Human HNRNPR were performed. The affinity constant is 0.868 nM.

HNRNPR Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85078-5-PBS

Clone No.242734A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242734A8, hnRNP R, HNRPR

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Product Information

85078-5-PBS targets HNRNPR in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNRNPR fusion protein Ag30339
• Full Name: heterogeneous nuclear ribonucleoprotein R
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 65-82 kDa
• GenBank Accession Number: BC001449
• Gene Symbol: HNRNPR
• Gene ID (NCBI): 10236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43390
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)